Phospholipase

Class of enzymes that cleave phospholipids
Phospholipase cleavage sites. An enzyme that displays both PLA1 and PLA2 activities is called a Phospholipase B.

A phospholipase is an enzyme that hydrolyzes phospholipids[1] into fatty acids and other lipophilic substances. Acids trigger the release of bound calcium from cellular stores and the consequent increase in free cytosolic Ca2+, an essential step in calcium signaling to regulate intracellular processes.[2] There are four major classes, termed A, B, C, and D, which are distinguished by the type of reaction which they catalyze:

  • Phospholipase A
    • Phospholipase A1 – cleaves the sn-1 acyl chain (where sn refers to stereospecific numbering).
    • Phospholipase A2 – cleaves the sn-2 acyl chain, releasing arachidonic acid.
  • Phospholipase B – cleaves both sn-1 and sn-2 acyl chains; this enzyme is also known as a lysophospholipase.
  • Phospholipase C – cleaves before the phosphate, releasing diacylglycerol and a phosphate-containing head group. PLCs play a central role in signal transduction, releasing the second messenger inositol triphosphate.
  • Phospholipase D – cleaves after the phosphate, releasing phosphatidic acid and an alcohol.

Types C and D are considered phosphodiesterases.

Endothelial lipase is primarily a phospholipase.[3]

Phospholipase A2 acts on the intact lecithin molecule and hydrolyzes the fatty acid esterified to the second carbon atom. The resulting products are lysolecithin and a fatty acid. Phospholipase A2 is an enzyme present in the venom of bees, blennies and viper snakes.[4]

See also

References

  1. ^ "phospholipase" at Dorland's Medical Dictionary
  2. ^ Molinari, Giuliano; Nervo, Elsa (2021-02-26). "Role of protons in calcium signaling". The Biochemical Journal. 478 (4): 895–910. doi:10.1042/BCJ20200971. ISSN 1470-8728. PMID 33635336.
  3. ^ Yu JE, Han SY, Wolfson B, Zhou Q (2018). "The role of endothelial lipase in lipid metabolism, inflammation, and cancer". Histology and Histopathology. 33 (1): 1–10. doi:10.14670/HH-11-905. PMC 5858721. PMID 28540715.
  4. ^ D. M. Vasudevan & S. Sreekumari, Textbook of Biochemistry (5th ed.)

Further reading

  • Tappia, Paramjit S. & Dhalla, Naranjan S. (Editors): Phospholipases in Health and Disease. Springer, 2014. ISBN 978-1-4939-0463-1 [Print]; ISBN 978-1-4939-0464-8 [eBook]

External links

Wikimedia Commons has media related to Phospholipases.
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3.1.1: Carboxylic
ester hydrolases3.1.2: Thioesterase3.1.3: Phosphatase3.1.4:
Phosphodiesterase3.1.6: SulfataseNuclease (includes
deoxyribonuclease
and ribonuclease)
3.1.11-16:
Exonuclease
Exodeoxyribonuclease
Exoribonuclease
3.1.21-31:
Endonuclease
Endodeoxyribonuclease
Endoribonuclease
either deoxy- or ribo-    
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Extracellular
Eicosanoids
Lysophospholipids
Steroids
Others
Intracellular
Nuclear receptor
Second messenger
Precursors
General
Steroids


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