Neoschizomer
Type of restriction enzymes
Neoschizomers are restriction enzymes that recognize the same nucleotide sequence as their prototype but cleave at a different site. In some special applications this is a very helpful feature.
For example:Prototype MaeII A^CGT produces DNA fragments with a 2-base 5' extension Neoschizomer TaiI ACGT^ produces DNA fragments with a 4-base 3' extension
Prototype ApaI GGGCC^C produces DNA fragments with a 4-base 3' extension Neoschizomer Bsp120I G^GGCCC produces DNA fragments with a 4-base 5' extension
There are also other pairs of neoschizomers.
Neoschizomers are a subset of isoschizomers.
References
- Rebase restriction enzyme database, http://rebase.neb.com/rebase/
See also
Wikidata has the property:
- neoschizomer (P4875) (see uses)
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Restriction modification system: restriction enzyme
- Isoschizomer
- Neoschizomer
- Isocaudomer
- TaqI
- Sau3A
- AluI*
- HaeIII*
- DpnII
- NlaIII
- HpaII
- EcoRII
- FokI
- AaaI
- BglII
- PovII*
- EcoRV*
- EcoRI
- BamHI
- HindIII
- XbaI
- XhoI
- SacI
- PstI
- NdeI
- NotI
- A
- Ba–Bc
- Bd–Bp
- Bsa–Bso
- Bsp–Bss
- Bst–Bv
- C-D
- E-F
- G-K
- L-N
- O-R
- S
- T-Z
* means cleavage produces blunt ends
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