CPSF6

Protein-coding gene in humans
CPSF6
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

3P5T, 3P6Y, 3Q2S, 3Q2T, 4B4N, 4U0A, 4U0B, 4WYM

Identifiers
AliasesCPSF6, CFIM, CFIM68, HPBRII-4, HPBRII-7, cleavage and polyadenylation specific factor 6, CFIM72
External IDsOMIM: 604979; MGI: 1913948; HomoloGene: 134126; GeneCards: CPSF6; OMA:CPSF6 - orthologs
Gene location (Human)
Chromosome 12 (human)
Chr.Chromosome 12 (human)[1]
Chromosome 12 (human)
Genomic location for CPSF6
Genomic location for CPSF6
Band12q15Start69,239,569 bp[1]
End69,274,358 bp[1]
Gene location (Mouse)
Chromosome 10 (mouse)
Chr.Chromosome 10 (mouse)[2]
Chromosome 10 (mouse)
Genomic location for CPSF6
Genomic location for CPSF6
Band10|10 D2Start117,180,578 bp[2]
End117,215,920 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • ganglionic eminence

  • bone marrow cells

  • Achilles tendon

  • ventricular zone

  • tonsil

  • left ovary

  • right ovary

  • appendix

  • sural nerve

  • endometrium
Top expressed in
  • superior cervical ganglion

  • hand

  • otolith organ

  • utricle

  • mandibular prominence

  • cumulus cell

  • trigeminal ganglion

  • maxillary prominence

  • abdominal wall

  • ganglionic eminence
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
  • protein binding
  • mRNA binding
  • RNA binding
  • nucleic acid binding
  • ribosomal large subunit binding
  • exon-exon junction complex binding
Cellular component
  • paraspeckles
  • membrane
  • nucleus
  • nucleoplasm
  • nuclear speck
  • mRNA cleavage factor complex
  • perichromatin fibrils
  • cytoplasm
  • mRNA cleavage and polyadenylation specificity factor complex
  • interchromatin granule
  • ribonucleoprotein complex
Biological process
  • mRNA polyadenylation
  • mRNA processing
  • protein tetramerization
  • positive regulation of RNA export from nucleus
  • protein heterotetramerization
  • pre-mRNA cleavage required for polyadenylation
  • mRNA alternative polyadenylation
  • messenger ribonucleoprotein complex assembly
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

11052

432508

Ensembl

ENSG00000111605

ENSMUSG00000055531

UniProt

Q16630

Q6NVF9

RefSeq (mRNA)

NM_001300947
NM_007007

NM_001013391
NM_001310609

RefSeq (protein)

NP_001287876
NP_008938

NP_001013409
NP_001297538

Location (UCSC)Chr 12: 69.24 – 69.27 MbChr 10: 117.18 – 117.22 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.[5][6][7]

Function

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.[7]

Interactions

CPSF6 has been shown to interact with WWP1[8] and PLSCR1.[8]

CPSF6 plays an important role in the nuclear import and integration of HIV-1 capsids.[9]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000111605 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000055531 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Rüegsegger U, Blank D, Keller W (Jan 1998). "Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits". Molecular Cell. 1 (2): 243–53. doi:10.1016/S1097-2765(00)80025-8. PMID 9659921.
  6. ^ Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SM (Apr 2007). "Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit". Molecular Biology of the Cell. 18 (4): 1282–92. doi:10.1091/mbc.E06-09-0846. PMC 1838998. PMID 17267687.
  7. ^ a b "Entrez Gene: CPSF6 cleavage and polyadenylation specific factor 6, 68kDa".
  8. ^ a b Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  9. ^ Müller TG, Zila V, Müller B, Kräusslich HG (29 September 2022). "Nuclear Capsid Uncoating and Reverse Transcription of HIV-1". Annual Review of Virology. 9 (1): 261–284. doi:10.1146/annurev-virology-020922-110929. PMID 35704745.

External links

Further reading

  • Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
  • Rüegsegger U, Beyer K, Keller W (Mar 1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors". The Journal of Biological Chemistry. 271 (11): 6107–13. doi:10.1074/jbc.271.11.6107. PMID 8626397.
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
  • de Vries H, Rüegsegger U, Hübner W, Friedlein A, Langen H, Keller W (Nov 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". The EMBO Journal. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040.
  • Awasthi S, Alwine JC (Nov 2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA. 9 (11): 1400–9. doi:10.1261/rna.5104603. PMC 1287061. PMID 14561889.
  • Brown KM, Gilmartin GM (Dec 2003). "A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im". Molecular Cell. 12 (6): 1467–76. doi:10.1016/S1097-2765(03)00453-2. PMID 14690600.
  • Dettwiler S, Aringhieri C, Cardinale S, Keller W, Barabino SM (Aug 2004). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization" (PDF). The Journal of Biological Chemistry. 279 (34): 35788–97. doi:10.1074/jbc.M403927200. PMID 15169763. S2CID 39752996.
  • Venkataraman K, Brown KM, Gilmartin GM (Jun 2005). "Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition". Genes & Development. 19 (11): 1315–27. doi:10.1101/gad.1298605. PMC 1142555. PMID 15937220.
  • Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  • Millevoi S, Loulergue C, Dettwiler S, Karaa SZ, Keller W, Antoniou M, Vagner S (Oct 2006). "An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries". The EMBO Journal. 25 (20): 4854–64. doi:10.1038/sj.emboj.7601331. PMC 1618107. PMID 17024186.
  • Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
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